Coding

Part:BBa_K3084000:Design

Designed by: Lucas Coppens   Group: iGEM19_KU_LEUVEN   (2019-10-15)


Venus Yellow Fluorescent Protein (YFP), S-TIP37 codon optimised


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 424
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our team looked more closely into the genome of S-TIP37 phage to be able to identify the capsid protein as a strongly expressed protein. We decided to take over the codon distribution found on the phage genome in our design.


Source

Part:BBa_K592101 from the iGEM registry

References